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Cell & Cellular Life Sciences Journal Research Article 1 min read

Evidence of CR Receptor Gene in an Invertebrate

Leclerc M* and Kresdorn N*
* Corresponding author
ISSN: 2578-4811  10.23880/cclsj-16000105  Received: December 13, 2016  Published: December 27, 2016
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Keywords
CR receptor gene Sea star primitive antibody Invertebrate
Abstract

In 2013, Complement components were found in the Asterias rubens genome, when compared to mouse genome (Classical and alternative components). Recently, we discovered a Fab gene and Fc gene in the A.rubens transcriptome. A link was missing: the CR receptor gene which is described in the present paper.

Introduction

All the elements which constitute a primitive antibody were discovered in the sea star genome [1] but the CR receptor gene was missing. Nevertheless, Complement components were shown in 2013 [2] especially the C1q component which can link, in mammals to the Fc site of Immunoglobulins. We attempt, in the present paper, to analyse the sea star CR receptor gene.

Materials and Methods

Sea stars were obtained from the Biology Institute (Gothenburg University). Immunizations to HRP, genomic studies, were already described [2]. After ligation of adapters for Illumina's GSII sequencing system, the cDNA was sequenced on the Illumina GSII platform sequencing. 1.100 bp from one side of the approximately 200 bp fragments sequences were assembled using Velvet [3].

Results

One counting (c20648_g1_i1) could be annotated via BLASTX to Mus musculus "Complement component C1q receptor" from the Uniprot database (sp|O89103|C1QR1_MOUSE), with an e-value of 1e-09. On an aligned region of 83 amino acids, 44 positive and 34 identical amino acids were found. 5'GGATACACTCTGGACCCTGATCGACATGCTTGCAATAATA TTGATGAATGCTCAATGACT CGCACCCACAACTGTCACCAGAATTGCACTGATACCCCCGG TAGCTACAGCTGTAGTTGT TACTCTGGTTACTCGCTAGTGGATCCCAAAGGAGTCGAGTG TGGTGACTATGACGAATGT GAAAAGAAGGCGGATAACGAGTGTGAACAGCTTTGCATGA ACTTGGAGGGATTTTACAGC TGTGATTGCTACCCCGGGTATGAG3'

Discussion and Conclusion

Cell & Cellular Life Sciences Journal

References

  1. Leclerc M, Kresdorn N (2016) The Fc Receptor Gene in an Invertebrate. EC Microbiology 3(6): 605-607.
  2. Leclerc M, Nicolas Kresdornb, Björn Rotterb (2013) Evidence of complement genes in the sea-star Asterias rubens. Comparisons with the sea urchin. Immunol Lett 151: 68-70.
  3. Zerbino DR, et al. (2007) Gen Res 18: 821-829.
  4. Vincent N, Osterasa M, Ottena P, Leclerc M (2014) A new gene in A rubens: A sea star Ig kappa gene Metagene 2: 320-322.
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@article{leclerc2016,
  title   = {Evidence of CR Receptor Gene in an Invertebrate},
  author  = {Leclerc M* and Kresdorn N},
  journal = {Cell & Cellular Life Sciences Journal},
  year    = {2016},
  volume  = {1},
  number  = {1},
  doi     = {10.23880/cclsj-16000105}
}
Leclerc M* and Kresdorn N (2016). Evidence of CR Receptor Gene in an Invertebrate. Cell & Cellular Life Sciences Journal, 1(1). https://doi.org/10.23880/cclsj-16000105
TY  - JOUR
TI  - Evidence of CR Receptor Gene in an Invertebrate
AU  - Leclerc M* and Kresdorn N
JO  - Cell & Cellular Life Sciences Journal
PY  - 2016
VL  - 1
IS  - 1
DO  - 10.23880/cclsj-16000105
ER  -