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Gastroenterology & Hepatology International Journal Research Article 1 min read

Organoid Culture and Its Importance

Aditi Banerjee*
* Corresponding author
ISSN: 2574-8009  10.23880/ghij-16000122  Received: July 12, 2017  Published: July 14, 2017
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Acknowledgements

Thanks to Andrea Bafford, MD for obtaining surgical colon tissue and Tamaki Ikuse, MD for assistant in dissection of colon biopsy material. I also wish to thank the Department of Pediatrics at the University of Maryland School Of Medicine for supporting this research.

Figure 1: Human colon organoid. Tissues were obtained from The University of Maryland School of Medicine with informed consent and the study was approved by the ethical committee. The isolation of healthy crypts was performed essentially as described by Sato et al [5].
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Figure 1: Human colon organoid. Tissues were obtained from The University of Maryland School of Medicine with informed consent and the study was approved by the ethical committee. The isolation of healthy crypts was performed essentially as described by Sato et al [5].

References

  1. van de Wetering M, Francies HE, Francis JM, Bounova G, Iorio F, et al. (2015) Prospective derivation of a living organoid biobank of colorectal cancer patients. Cell 161: 933-945.
  2. Meneses AMC, Schneeberger K, Kruitwagen HS, Penning LC, van Steenbeek FG, et al. (2016) Intestinal organoids-current and future applications. Veterinary Sciences 3(4): 31.
  3. Banerjee A, Ahmed H, Yang P, Czinn SJ, Blanchard TG (2016) Endoplasmic reticulum stress and IRE-1 signaling cause apoptosis in colon cancer cells in response to andrographolide treatment. Oncotarget 7(27): 41432-41444.
  4. Banerjee A, Banerjee V, Czinn S, Blanchard T (2017) Increased reactive oxygen species levels cause ER stress and cytotoxicity in andrographolide treated colon cancer cells. Oncotarget 8(16): 26142-26153.
  5. Sato T, Stange DE, Ferrante M, Vries RG, Van Es JH, et al. (2011) Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium. Gastroenterology 141(5): 1762-1772.
  6. Broutier L, Andersson-Rolf A, Hindley CJ, Boj SF, Clevers H, et al. (2016) Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation. Nature Protocol 11(9): 1724-1743.
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@article{aditi2017,
  title   = {Organoid Culture and Its Importance},
  author  = {Aditi Banerjee},
  journal = {Gastroenterology & Hepatology International Journal},
  year    = {2017},
  volume  = {2},
  number  = {2},
  doi     = {10.23880/ghij-16000122}
}
Aditi Banerjee (2017). Organoid Culture and Its Importance. Gastroenterology & Hepatology International Journal, 2(2). https://doi.org/10.23880/ghij-16000122
TY  - JOUR
TI  - Organoid Culture and Its Importance
AU  - Aditi Banerjee
JO  - Gastroenterology & Hepatology International Journal
PY  - 2017
VL  - 2
IS  - 2
DO  - 10.23880/ghij-16000122
ER  -